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Organotypic mind slices are an in vitro model that replicates in vivo body structure with additional throughput and optical advantages, therefore pairing well with microfluidic devices. Brain slices have advantages over primary cell way of life in that tissue architecture is preserved and multicellular interactions can still occur. There is flexibility in their use, as slices can be used acutely (much less than 6 hours after slice harvesting) or cultured for later experimental use. Because organotypic mind slices can maintain viability for weeks, they allow for long-term results to be studied. Slice-primarily based systems additionally offer experimental get entry to with precise control of extracellular environments, making it a suitable platform for correlating disease with neuropathological outcomes. Because approximately 10 to 20 slices may be extracted from a single mind, animal utilization is drastically reduced relative to in vivo research. Organotypic brain slices may be extracted and cultured from a couple of animal species (e.G. Rats), but additionally from people