Agar gel method

Agar gel method
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  • Media solidified with agar are also used for organ culture and those media include 7 parts 1% agar in BSS, 3 elements chick embryo extract and three components of horse serum. Defined media with or without serum are also used with agar. The medium with agar gives the mechanical aid for organ culture. It does not liquefy. Embryonic organs typically grow properly on agar, but adult organ tradition will not live to tell the tale on this medium.
  • The lifestyle of person organs or components from adult animals is extra difficult because of their greater requirement of oxygen. A style of person organs (e.G. The liver) had been cultured using unique media with unique apparatus (Towell’s II tradition chamber). Since serum turned into observed to be poisonous, serum-unfastened media were used, and the special apparatus approved the use of 95% oxygen.

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